Daptomycin-resistant Staphylococcus capitis: mechanisms, molecular epidemiology, and clinical aspects.

Authors

Junxiong Zhang, Zhengan Wang, Chao Gu, Yueqin Hong, Yiyi Chen, Feiteng Zhu, Mengzhen Chen, Hemu Zhuang, Haiping Wang, Lu Sun, Yan Chen, Shengnan Jiang

Published in

Journal of global antimicrobial resistance. Jun 24, 2026. Epub Jun 24, 2026.

Abstract

Methicillin-resistant coagulase-negative staphylococci are important nosocomial pathogens. Studies on daptomycin (DAP) resistance in coagulase-negative staphylococci focus on Staphylococcus epidermidis, but few on Staphylococcus capitis despite its clinical and epidemiological importance. This study investigates the DAP susceptibility and underlying resistance mechanisms of methicillin-resistant S. capitis (MRSC) isolated from patients.
Clinical MRSC strains collected in China between 2018 and 2020 were analyzed. The minimum inhibitory concentrations (MICs) were determined by agar dilution and broth microdilution methods. Molecular typing of S. capitis was performed using multilocus sequence typing. Whole-genome sequencing was performed to analyze mutations between DAP-resistant and -susceptible strains, which were further validated by phenotypic assays.
A total of 101 MRSC strains were analyzed, of which 99.01% (100/101) were DAP-susceptible, and 48.51% (49/101) exhibited reduced susceptibility (MIC > 0.5 µg/mL)-including 1 strain (0.99%) that was resistant (MIC = 4 µg/mL). ST1 (38.61%), ST6 (35.64%) and ST3 (6.93%) were dominant. ST6 strains were significantly more susceptible to DAP than ST1 (p < 0.001). No cross-resistance was observed between DAP and vancomycin (p = 0.604) or teicoplanin (p = 0.143). In the DAP-resistant strain, two novel mutations (mprF-A784L, yycF-H120P) were identified. Complementation of mutant mprF restored DAP resistance (MIC = 2 µg/mL) while increasing susceptibility to β-lactams. This "seesaw effect" provides valuable insights for the treatment of MRSC infections.
This study provides in vitro DAP activity data for S. capitis and identifies novel mprF mutations affecting its susceptibility to DAP and β-lactams.

PMID:
42342121
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.

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