Authors
Morgan A Esler, Rachel Werther, Lindsey A Doyle, Natalia C Ubilla-Rodriguez, Jeanette S Schwensen, Jazmine P Hallinan, Abigail R Lambert, Juliana C Young, Miriam Silverstein, Barry L Stoddard
Published in
Nucleic acids research. Volume 54. Issue 12. Jun 22, 2026.
Abstract
The recent development of AI-based predictive tools, such as AlphaFold3, for the prediction of the structures of biological molecules and their complexes has transformed modern molecular and cellular biology. While it displays exceptional accuracy in the modeling of folded protein domains and subunits, as well as larger protein-protein complexes and assemblages, AlphaFold3's performance in predicting the details of protein-DNA (or more broadly, protein-nucleic acid) contacts and complexes is less well established. Here we summarize the recent development and performance of tools intended to predict, model, and/or design protein:DNA recognition and contacts, and then demonstrate (using a well-defined system that offers a minimal "degree of difficulty") the issues that often surround the use of a resource such as AlphaFold3 for predicting protein:DNA interactions. Beyond providing a cautionary tale for casual users, we note that the incorporation of hybrid models of protein-DNA complexes (in which computationally predicted models are docked into low-resolution CryoEM density maps with little further refinement or quality control) into future training sets may lead to an ongoing and inappropriate learning cycle that further encourages such tools to generate new, equally inaccurate models of protein-DNA complexes.
PMID:
42345194
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.
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