Authors
Jinsuo Li, Hang Su, Gengchen Xi, Xueqing Shi, Guangrong Hu, Fuli Li, Ming Lyu
Published in
Sheng wu gong cheng xue bao = Chinese journal of biotechnology. Volume 42. Issue 6. Pages 2644-2660. Jun 25, 2026.
Abstract
To meet the demand for CO2 resource utilization under the "dual-carbon" goals, the application of medium optimization combined with fermentation process regulation to enhance the acetate production and gas utilization capacity of Acetobacterium woodii represents an effective process optimization strategy. In this study, a multi-strategy approach combining medium optimization and fermentation process regulation was adopted to enhance the acetate yield of an A. woodii SL70 strain obtained via adaptive laboratory evolution (ALE). Firstly, through single-factor experiments and response surface methodology, the optimal culture conditions were determined as follows: gas ratio H2:CO2=70:30, NaCl 5 g/L, and yeast extract 4 g/L. Further fermentation process adjustments were performed in a 10 L bioreactor. By increasing the gas flow rate from 1 L/min to 2 L/min and raising the headspace pressure from 1.2 bar to 1.5 bar, the acetate concentration was elevated from 19.18 g/L to 41.87 g/L, representing a 118.3% increase in yield. These modifications also significantly improved the synergy between biomass growth and product synthesis, as well as fermentation stability. This study provides an efficient fermentation medium formulation and process parameters for the industrial application of A. woodii, offering technical support for the directed conversion of CO2 into high-value acetate products.
PMID:
42343803
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.
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