[Heterologous biosynthesis and production optimization of p-coumaric acid in Streptomyces coelicolor].

Authors

Jinyan Ding, Xueru Feng, Wendi Zheng, Haotian Liu, Jie Gao, Meng Wang, Yue Zhang

Published in

Sheng wu gong cheng xue bao = Chinese journal of biotechnology. Volume 42. Issue 6. Pages 2521-2537. Jun 25, 2026.

Abstract

p-coumaric acid is an important bioactive natural product and a crucial precursor for the synthesis of downstream phenylpropanoids. Compared with plant extraction and chemical synthesis methods, microbial production of p-coumaric acid offers unique advantages, including environmental friendliness, short production cycles, and high conversion efficiency. Streptomyces species possess a complete shikimate pathway and an extensive aromatic amino acid metabolic network, being promising chassis for the production of p-coumaric acid. This study aims to achieve efficient heterologous biosynthesis of p-coumaric acid in Streptomyces coelicolor through heterologous expression of tyrosine ammonia-lyase genes from diverse sources, combined with fermentation condition optimization and precursor supplementation strategies. Streptomyces coelicolor M145 was used as the chassis for the heterologous expression of two tyrosine ammonia-lyase genes, ScTAL from S. clavuligerus and RcTAL from Rhodobacter capsulatus, successfully achieving the heterologous biosynthesis of p-coumaric acid. Through fermentation medium optimization, the use of TSB (dextrin) medium effectively resolved the autolysis of mycelia in the late fermentation stage, balancing cell growth and product synthesis and resulting in a p-coumaric acid titer of 25.07 mg/L. On this basis, exogenous supplementation of l-tyrosine precursor further enhanced the p-coumaric acid production capacity per cell by 1.53 folds, with a shake-flask fermentation titer reaching 27.27 mg/L. This study demonstrated the potential of S. coelicolor as a chassis for p-coumaric acid production, laying a foundation for efficient synthesis of high-value downstream phenylpropanoid natural products in Streptomyces.

PMID:
42343795
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.

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