Anion-Dependent Modulation of Cytochrome c Structural Dynamics in Bmim-Based Ionic Liquid-Water Systems.

Authors

Shalini Yadav, Arnab Sil, Renu Bhati, Kshatresh Dutta Dubey, Biswajit Guchhait

Published in

The journal of physical chemistry. B. Jun 25, 2026. Epub Jun 25, 2026.

Abstract

The stability and functionality of biomolecules in ionic liquid (IL) environments have garnered significant interest due to their potential applications in biocatalysis, pharmaceutical formulations, and, particularly, protein preservation. In this study, we systematically investigated the effect of three Bmim-based IL-water systems, [BmimAcO-water], [BmimDCA-water], and [BmimPF₆-water], on the stability of cytochrome c (Cyt-C) using molecular simulation tools. Our study reveals a significant impact on the structural stability of cytochrome c in different ionic liquids. We show that [BmimAcO-water] is the most suitable solvent for Cyt-C, and it preserves its structural topology better than the other two ionic liquid complexes. In contrast, the [BmimPF₆-water] system showed the highest destabilization, particularly near the heme cofactor. The [BmimDCA-water] system exhibited intermediate behavior, with structural stability metrics intermediate between those of the AcO^- and PF₆^- systems. Taken together, these findings highlight the pivotal influence of IL composition on the protein structure and dynamics.

PMID:
42345044
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.