Identification of Ferroptosis-related Genes Associated with the Prognosis of Hepatocellular Carcinoma.

Authors

Kai Yan, Fangfang Hu, Haodong Tang, Jiahua Zhou

Published in

Cell biochemistry and biophysics. Jun 25, 2026. Epub Jun 25, 2026.

Abstract

Ferroptosis has been increasingly implicated in the progression of various tumors, including hepatocellular carcinoma (HCC). This study aimed to identify ferroptosis-related genes with prognostic significance in HCC. Differential expressed genes (DEGs) and long non-coding RNAs (DElncRNAs) were identified from TCGA LIHC RNA-seq data. A competitive endogenous RNA (ceRNA) network was established based on ferroptosis-related lncRNA-mRNA pairs. Prognostic genes were identified through survival analysis, and selected DEGs were further validated via quantitative PCR. Co-expression analysis identified 218 lncRNA-mRNA pairs, including 216 DEGs and 11 DElncRNAs. Among these, 11 upregulated ferroptosis-related genes were identified as predicted targets of the lncRNA DDX11-AS1. MiR-195 and miR-424 both regulated CDC25A and HELLS. Elevated expression of DDX11-AS1, CDC25A, EZH2, FANCD2, and HELLS was associated with poorer survival. In vitro cellular experiments showed that the knockdown of DDX11-AS1, CDC25A, and HELLS inhibited cell proliferation and invasion by promoting ferroptosis in Huh7 cells. The 11 ferroptosis-related genes and DDX11-AS1 may serve as valuable prognostic biomarkers and potential immunotherapeutic targets in HCC.

PMID:
42348113
Bibliographic data and abstract were imported from PubMed on 25 Jun 2026.

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