Authors
Zhengwei Tan, Lei Li, Yan Liang, Chunming Li, Xiaoyu Su, Dandan Lu, Yao Sun, Lina Wang, Mengfan Su, Yiwen Cao, Huizhen Liang
Published in
Current issues in molecular biology. Volume 48. Issue 1. Dec 21, 2025. Epub Dec 21, 2025.
Abstract
Origanum vulgare L., a medicinal herb rich in bioactive phenols and terpenes, is recognized for its anti-inflammatory and antimicrobial properties. Heavy-ion beam mutagenesis, a sophisticated breeding technique, can induce significant variations in plants, thereby affecting their secondary metabolite production. This study utilized metabolomic and transcriptomic approaches to investigate the effects of 12C6+ heavy-ion irradiation on oregano. Our results indicated substantial changes in mutant lines, including marked alterations in plant height, leaf morphology, and biomass accumulation. Metabolomic analysis indicated that the differentially accumulated volatile compounds were primarily terpenoids. Furthermore, transcriptomic analysis indicated a predominant enrichment of differentially expressed genes in terpenoid biosynthesis. Integrated analyses identified key transcriptional changes in genes encoding terpenoid backbone enzymes, such as GPPS, GGPPS, DXS, and HMGR, and pinpointed candidate genes, including TPS3, TPS6A, TPS6C, CYP71D178, CYP71D181, and CYP71D10B, whose expression patterns were closely associated with the differential accumulation of carvacrol and thymol. This comprehensive study elucidates the molecular mechanisms underlying metabolic reprogramming induced by heavy-ion irradiation in oregano and offers valuable genetic resources for future metabolic engineering and precision breeding initiatives aimed at enhancing the production of valuable bioactive compounds.
PMID:
41614838
Bibliographic data and abstract were imported from PubMed on 26 Jun 2026.
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