Structural characterization, controlled enzymatic hydrolysis and immunomodulatory activities of three ginseng polysaccharides.

Authors

Zhenlei Wang, Huizhong Ma, Yuhan Zhang, Yubi Wang, Haitao Fan, Jiwei Shen, Jialin Jiang, Yufeng Liu

Published in

Carbohydrate research. Volume 567. Pages 110021. Jun 26, 2026. Epub Jun 26, 2026.

Abstract

This study systematically investigated the structure-immunomodulatory activity relationship of three ginseng polysaccharides (GPs). GP40015-1 was identified as a high-molecular-weight neutral glucan, whereas GP40025-1 and GP40030-1 were acidic heteropolysaccharides with distinct uronic acid contents and branching patterns. Controlled enzymatic hydrolysis combined with LC-ESI-MS/MS was employed to decipher their specific oligosaccharide sequences. Immunomodulatory assessment on RAW264.7 macrophages demonstrated that all three GPs promoted cell proliferation and phagocytic activity in a dose-dependent manner. GP40025-1 exhibited the strongest bioactivity, enhancing proliferation by 158.4% and phagocytosis to 246.6% at 100 μg/mL. Its superior immunomodulatory potential was attributed to a lower molecular weight, a unique β-linked backbone with →1)-α-Glc-(3→ and →1)-β-GlcA-(4→ branching motifs, and an optimal uronic acid content. These findings provide crucial insights into the structure-activity relationship of GPs and underscore the potential of GP40025-1 as a functional food ingredient with natural immunomodulatory properties.

PMID:
42364270
Bibliographic data and abstract were imported from PubMed on 28 Jun 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.