Targeting delivery systems with aptamer-conjugated hyaluronic acid for personalized assessment of treatment response to targeted cancer therapy.

Authors

Qing-Yu Gao, Di Han, Xin-Ru Liao, Jin-Ju Lei, Li-Jin Qi, Qi-Yang Huang, Fei-Fei Fu, Si-Xue Cheng

Published in

International journal of biological macromolecules. Pages 153260. Jun 27, 2026. Epub Jun 27, 2026.

Abstract

A central objective in oncology is to identify therapeutic targets essential for the survival of proliferating malignant cells. However, a major challenge in pre-clinical research is the scarcity of robust models capable of accurately assessing therapeutic efficacy in patient-specific heterogeneous cancer cells. Here, we developed an ex vivo platform for patient-specific assessment using biomacromolecule-based targeting delivery systems for CRISPR-Cas9 mediated gene knockout and in situ mRNA profiling in patient-derived circulating malignant cells (CMCs). Using a cancer targeting vector, we conducted a personalized evaluation of the impact of Rac GTPase activating protein 1 (RACGAP1) knockout on cell growth in a cancer cell line and patient-derived heterogeneous CMCs. RACGAP1 knockout induced irreversible cytokinesis failure, leading to markedly reduced proliferation and invasion capacity, DNA damage, and increased apoptosis. Our approach establishes a promising strategy for the personalized validation of novel therapeutic targets.

PMID:
42364748
Bibliographic data and abstract were imported from PubMed on 28 Jun 2026.

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