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A Low-Cost Metabolic Apparatus for Student Labs: Measuring Oxygen Consumption and Ventilation in Conscious Mice.

Created on 29 Jun 2026

Authors

Yan Liu, Jia Lin, Wenzhen Yu, Chunxiang Tu, Xingui Zhang, Ling Chen, Wenrong Shi

Published in

Advances in physiology education. Jun 29, 2026. Epub Jun 29, 2026.

Abstract

Hands-on experience in measuring core physiological parameters like oxygen consumption (VO₂) is essential for deep conceptual understanding, yet the prohibitive cost of commercial systems often excludes such experiments from science curricula. To bridge this gap, we developed and implemented a ready-to-use educational module centered on a simple, low-cost metabolic apparatus. The apparatus, constructed from commercially available components, enables direct measurement of VO₂ and respiratory parameters in conscious mice by students. Moreover, the apparatus was successfully used to detect and quantify the opposing physiological effects of two classic metabolic-modulating drugs: levothyroxine sodium (increasing VO₂ and minute ventilation) and chlorpromazine hydrochloride (decreasing VO₂, respiratory frequency, and ventilation). This module transforms abstract principles of energy metabolism, respiratory control, and pharmacology into an investigable, inquiry-based learning experience. The apparatus features straightforward assembly and operation, and is accompanied by a comprehensive instructional manual. These attributes render it particularly well suited for integration into undergraduate and graduate physiology and pharmacology laboratories, thereby broadening access to advanced physiological measurement techniques.

PMID:
42370859
Bibliographic data and abstract were imported from PubMed on 29 Jun 2026.

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