K38 Succinylation of CsrA Regulates Biofilm Formation in Pseudomonas fluorescens PF08 by Modulating c-di-GMP Levels.

Authors

Feifei Wang, Runyu Yang, Zhuoer Chen, Rong Tan, Jian Chen, Ruiyu Zhu, Yanbo Wang

Published in

Journal of agricultural and food chemistry. Jul 02, 2026. Epub Jul 02, 2026.

Abstract

Pseudomonas fluorescens PF08 is a significant food spoilage organism belonging to a species widely utilized for agricultural biocontrol, yet the post-translational regulation of its biofilm formation remains poorly understood. This work explored the role of lysine succinylation (Ksucc) in regulating CsrA activity, a global post-transcriptional regulator. NanoHPLC-MS/MS identified Lys38 (K38), a highly conserved residue within the CsrA RNA-binding interface, as a functional succinylation site. Site-directed mutagenesis revealed that K38 succinylation significantly inhibits biofilm formation by reducing c-di-GMP levels, exopolysaccharide production, and surface hydrophobicity, while enhancing flagellar-mediated motility. Colonization assays on foods, contact surfaces, and the rhizosphere confirmed that CsrA succinylation restricts biofilm formation across diverse ecological niches. These results identify K38 succinylation of CsrA as a regulatory modification that modulates the transition between motile and sessile lifestyles in P. fluorescens PF08. These findings expand the understanding of post-translational control of biofilm formation in this ecologically versatile bacterium relevant to food spoilage and agricultural environments.

PMID:
42391643
Bibliographic data and abstract were imported from PubMed on 03 Jul 2026.

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