A caspase-3-activated protein expression system for apoptosis visualization and apoptosis-pyroptosis conversion to boost antitumor activity.

Authors

Wen-Jie Shu, Chunxia He, Zhe Ma, Lijun Jia, Bin Guo, Pengfei Chen, Fu Wang

Published in

Trends in biotechnology. Jul 02, 2026. Epub Jul 02, 2026.

Abstract

Dysregulation of cell death is a hallmark of cancer, and overcoming tumor resistance to apoptosis-inducing therapies remains challenging. Inducing immunogenic cell death (ICD), such as pyroptosis, is promising but currently lacks tools for in vivo monitoring and control. Here, we developed a caspase-3-activated, genetically encoded protein expression system-a programmable synthetic biology platform activated by the key apoptotic protease caspase-3-for apoptosis detection. Configured to express fluorescent or luciferase proteins, it enabled high-resolution live-cell apoptosis imaging and in vivo tumor bioluminescence imaging, and it served as a screening tool for proapoptotic microRNAs. Most importantly, engineering it to express a pyroptosis effector triggered apoptosis-coupled pyroptosis, achieving potent cancer cell killing and enhanced in vivo antitumor efficacy. This versatile platform advances apoptosis detection and its conversion to potent ICD, offering an adaptable approach for cancer theranostics.

PMID:
42392927
Bibliographic data and abstract were imported from PubMed on 03 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.