Authors
Yu-Chen Zhu, Wei Liu, Zhen-Nan Jiang, Yuan Yuan
Published in
Zhongguo Zhong yao za zhi = Zhongguo zhongyao zazhi = China journal of Chinese materia medica. Volume 51. Issue 8. Pages 2211-2219.
Abstract
The dried root of Pueraria thomsonii, a plant of the Fabaceae family, features homology of medicine and food. It is rich in isoflavone glycosides such as puerarin and daidzin. Glycosylation, catalyzed by uridine diphosphate(UDP) glycosyltransferase(UGT), is a key post-modification step in isoflavone biosynthesis. However, the functional characterization of UGT in P. thomsonii remains limited. In this study, two putative O-glycosyltransferase genes, PtUGT-6 and PtUGT-7, were identified from the P. thomsonii transcriptome. Phylogenetic tree analysis revealed that PtUGT-6 and PtUGT-7 clustered closely with PlUGT15, an isoflavone 4',7-O-glycosyltransferase from P. lobata. In vitro enzymatic assays demonstrated that both PtUGT-6 and PtUGT-7 catalyzed the conversion of daidzein to daidzin. Enzymatic property analysis showed that the optimal reaction temperatures of PtUGT-6 and PtUGT-7 were 37 and 45 ℃, respectively, with an optimal pH of 9.0 for both enzymes. Furthermore, no metal ion dependence was exhibited. After 1 hour of the reaction, the substrate conversion rate reached approximately 80% and then tended to stabilize. Kinetic analysis revealed that the K_m values of PtUGT-6 and PtUGT-7 were 22.43 and 29.39 μmol·L~(-1), respectively, with corresponding K_(cat) values of 2.16×10~(-3) and 2.17×10~(-3) s~(-1) and K_(cat)/K_m of 96.30 and 73.83 L·mol~(-1)·s~(-1), respectively. In summary, this study identified two glycosyltransferase genes, PtUGT-6 and PtUGT-7, from P. thomsonii, which can catalyze the glycosylation of daidzein to daidzin. These findings provide important insights into the biosynthetic pathway of characteristic isoflavone glycosides in P. thomsonii and offer a theoretical basis for improving the accumulation and quality of active compounds in this medicinal plant.
PMID:
42392708
Bibliographic data and abstract were imported from PubMed on 03 Jul 2026.
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