Inhibition of thrombospondin-1 signaling prevents experimental bronchopulmonary dysplasia.

Authors

Chantal Gaudet, Kathrine L Daniel, Sophie Holcik, Ali Hamraghani, Behzad Yeganeh, Robert P Jankov

Published in

American journal of physiology. Lung cellular and molecular physiology. Jul 03, 2026. Epub Jul 03, 2026.

Abstract

Bronchopulmonary dysplasia (BPD) is a neonatal lung injury characterized by inflammation, and alveolar and vascular hypoplasia that currently lacks effective treatment. Thrombospondin (TSP)-1 is an angiostatic and pro-inflammatory protein, recently implicated in BPD pathogenesis, that both activates transforming growth factor (TGF)-β1 and suppresses nitric oxide (NO) signaling. In order to gain further insight into the relative importance of downstream effects of TSP-1, our objective in a neonatal rat model of hyperoxia-intermittent hypoxia (H-IH)-induced lung injury was to compare effects of inhibiting: 1) TSP-1-mediated TGF-β1 activation alone (LSKL) or 2) global TSP-1 signaling (soluble CD47 receptor ligand trap; sCD47r). From postnatal days (PND) 1-21, rat pups were exposed to air or to H-IH (PND 1-7 85% O2, PND 7-14 60% O2 and PND 14-21 air with intermittent exposure to 10% O₂ for 10 min every 4 hours) while either receiving daily s.c. LSKL (20 mg/kg) or third daily sCD47r (3 mg/kg). Controls were treated with vehicle or were continuously exposed to normoxia. Exposure to H-IH increased lung contents of TSP-1 and active TGF-β1, and caused macrophage influx, alveolar and pulmonary vascular hypoplasia, and pulmonary hypertension (PH). Both strategies prevented H-IH-mediated effects on active TGF-β1 content, macrophage influx, abnormal lung morphology and PH, while only sCD47r increased lung NO content and signaling. These observations in a clinically-relevant model indicate that multiple strategies aimed at suppressing TSP-1 signaling are effective in preventing lung injury and that targeting TSP-1-mediated activation of TGF-β1 is sufficient to achieve these effects.

PMID:
42397154
Bibliographic data and abstract were imported from PubMed on 03 Jul 2026.

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