Partial submergence-induced adventitious root emergence in cucumber requires CsRBOHB-mediated ROS production.

Authors

Yingying Hu, Jiajia Hu, Jiatao Shen, Yafang Jin, Xianran Chen, Qingmin Xie, Xuewen Xu, Xuehao Chen, Xiaohua Qi

Published in

Plant physiology. Jul 03, 2026. Epub Jul 03, 2026.

Abstract

Emergence of adventitious roots (ARs) in partially submerged plants is a crucial morphological adaptation to flooding stress, although the underlying molecular mechanisms are not well understood. In the present research, we investigated the role of CsRBOHB-mediated reactive oxygen species (ROS) production in cucumber (Cucumis sativus L.). ROS specifically accumulated in the vascular tissues of partially submerged hypocotyls. Respiratory burst oxidase homologs (RBOHs) are enzymes that generate ROS. We found that a cucumber RBOH gene, CsRBOHB, was specifically induced in vascular cells by partial submergence. Knockout of CsRBOHB inhibited AR emergence, while overexpression of CsRBOHB enhanced it. Downregulation of an RBOHB homolog SlRBOHB in partially submerged tomato also inhibited AR emergence. Additionally, cucumber calcium-dependent protein kinase 2 (CsCDPK2) directly activated CsRBOHB in cucumber hypocotyls during AR emergence, and silencing it inhibited AR emergence under partially submerged conditions. These findings indicate that AR emergence in partially submerged cucumber is regulated by a CsCDPK-CsRBOHB pathway.

PMID:
42397970
Bibliographic data and abstract were imported from PubMed on 04 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.