Real-time imaging of transcriptional feedback in nonsense-mediated mRNA decay.

Authors

Md Dobirul Islam, Tamoghna Das, Robert H Singer, Hanae Sato

Published in

Science advances. Volume 12. Issue 27. Pages eaea5923. Jul 03, 2026. Epub Jul 03, 2026.

Abstract

Nonsense-mediated mRNA decay (NMD) is a translation-coupled mRNA decay pathway triggered by a premature termination codon (PTC). While in-frame stop codons are typically defined by cytoplasmic ribosomes, unexpected changes in transcription have been reported in genes containing PTCs. This observation suggests the possibility of PTC detection at the transcription site (TS), which has not been thoroughly investigated with high temporal and spatial resolution. Here, we use a real-time imaging approach to simultaneously detecting transcription sites (TSs) expressing wild-type or NMD-targeted β-globin reporter genes in the same cell. Our data indicate a dynamic change in the transcription of PTC-containing β-globin mRNA that depends on translation, NMD, and nuclear protein import, supporting the existence of rapid transcriptional feedback following NMD in the cytoplasm. This study establishes a robust temporal link between cytoplasmic mRNA decay and nuclear transcription.

PMID:
42397899
Bibliographic data and abstract were imported from PubMed on 04 Jul 2026.

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