Authors
Ruiwen Li, Hao Shen, Dandan Shi, Shaoning Yu, Guoqing Qian
Published in
Mikrochimica acta. Volume 193. Issue 8. Jul 03, 2026. Epub Jul 03, 2026.
Abstract
Rapid and reliable detection of multidrug-resistant bacteria is essential for effective clinical management. In this study, we have developed a dual-mode aptasensing platform based on a core-shell MOF-on-MOF structure (UiO-66-NH₂@MOF-919) for the subtype-specific detection of carbapenem-resistant Klebsiella pneumoniae strains carrying NDM-1 and KPC-2. This bifunctional system integrates the peroxidase-like activity of the MOF-919 shell with the fluorescence quenching capability of the UiO-66-NH₂ core, enabling both colorimetric and fluorometric detection within a single platform. Benefiting from this design, the method achieves sensitive and selective detection over a wide linear range from 10 to 10⁸ CFU mL⁻¹, with low detection limits of 7 CFU mL⁻¹ for NDM-1 KP and 4 CFU mL⁻¹ for KPC-2 KP. The proposed platform exhibits good selectivity against non-target bacteria and strong anti-interference capability. Spiked recovery experiments in human serum, urine, and bronchoalveolar lavage fluid yielded recoveries ranging from 93.0% to 110.0% with RSDs below 5.0%, demonstrating good analytical accuracy and applicability in complex biological samples. Compared to conventional aptasensing systems, this approach enables dual-subtype detection within a single platform, improving analytical efficiency and practicality. This work provides a feasible strategy for MOF-on-MOF-based biosensing systems and shows promising potential for rapid clinical detection of drug-resistant bacteria.
PMID:
42399479
Bibliographic data and abstract were imported from PubMed on 04 Jul 2026.
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