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Defective PdMo metallene with efficient catalase-like activity for sensitive immunoassay.

Created on 05 Jul 2026

Authors

Chuan Tian, Bin Yang, Miaolin Yin, Hongye Yan, Yiming Zhang, Lei Jiao, Congxiao Wang

Published in

Analytica chimica acta. Volume 1416. Pages 345762. Sep 22, 2026. Epub May 30, 2026.

Abstract

Nanozyme-involved biosensing platforms show great potential in the accurate detection of trace analytes. Nevertheless, the rational design of high-performance nanozymes remains a significant challenge. Overcoming this hurdle is crucial for developing advanced immunoassays and, ultimately, for the construction of portable point-of-care testing devices. We successfully synthesized ultrathin PdMo metallene nanosheets (d-PdMoene), featuring a high density of defects, exhibiting efficient catalase-like activity (CAT-like activity) capable of catalyzing H2O2 into H2O and O2. Theoretical calculations reveal that d-PdMoene exhibits significant charge redistribution and upward shift of the d-band center, suggesting a superior electronic configuration for catalysis and enhancement of H2O2 adsorption. Besides, compared to PdMoene, a weaker bonding interaction between O2∗ and the d-PdMoene promotes easier O2 release. The generated O2 induces noticeable pressure changes, which can be easily monitored by a portable pressure gauge. As a concept application, an immunoassay sensor for prostate specific antigen was established, demonstrating high sensitivity and selectivity. Beyond the immediate outcomes, this study carries broader implications. It establishes a robust framework for the rational design of high-performance nanozymes and, concurrently, outlines a practical pathway toward the realization of portable testing devices.

PMID:
42401468
Bibliographic data and abstract were imported from PubMed on 05 Jul 2026.

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