A non-enzymatic sensor based on rGO/Pt NPs/Fc-Tyr/POPD nanocomposite for hydrogen peroxide determination in liver cancer tissues.

Authors

Xixiang Xie, Rongping Huang, Tao Zhao, Yaru Zhang, Rongfang Wang, Xiyu Liu, Qicai Xiao, Jian He, Pan Wu, Yong Huang

Published in

Mikrochimica acta. Volume 193. Issue 8. Jul 06, 2026. Epub Jul 06, 2026.

Abstract

Malignant tumors remain a major global health challenge, highlighting the need for rapid and sensitive analytical tools for investigating cancer-associated oxidative stress. In this study, we developed a non-enzymatic electrochemical hydrogen peroxide (H₂O₂) sensor based on a reduced graphene oxide/platinum nanoparticle/ferrocene-tyramine/poly(o-phenylenediamine) (rGO/Pt NPs/Fc-Tyr/POPD) nanocomposite. H₂O₂, although not a tumor-specific biomarker, is an important reactive oxygen species associated with oxidative stress in cancer-related systems. The proposed sensing platform integrates the high conductivity of rGO, the electrocatalytic activity of Pt NPs, the TSA-derived Fc-Tyr deposition strategy, and the anti-interference properties of POPD. The sensor exhibited two wide linear detection ranges (5.0 µM-570 µM and 570 µM-10.5 mM), a low detection limit of 1.18 µM, and a rapid response time of less than 5 s. In addition, the sensor demonstrated satisfactory selectivity, reproducibility, and stability. Compared to previously reported rGO/Pt-based H₂O₂ sensors, the present platform introduces a TSA-derived Fc-Tyr deposition strategy to facilitate interfacial electron transfer and increase the density of redox-active species. The sensor was successfully applied to monitoring H₂O₂ changes in cultured cancer cells and ex vivo tumor tissue-derived samples under chemically stimulated oxidative conditions. These findings suggest that the proposed system may provide a useful tool for investigating oxidative-stress-associated biological processes and electrochemical H₂O₂ determination.

PMID:
42406127
Bibliographic data and abstract were imported from PubMed on 06 Jul 2026.

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