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The scaffolding protein AKAP79/150 shapes innate immune responses to allergen.

Created on 07 Jul 2026

Authors

Suneet Kaur, Yu-Ping Lin, Hideki Nakano, Paula Brown, Rajesh Bhardwaj, Abdull J Massri, Hamed Bostan, Rajesh K Dutta, Kevin Gerrish, Donald N Cook, Anant B Parekh

Published in

Journal of immunology (Baltimore, Md. : 1950). Volume 215. Issue 6. Jun 07, 2026.

Abstract

Inhalation of house dust mite-derived allergens is a major factor leading to the development of asthma both in children and adults. Allergens stimulate Ca2+-dependent pro-inflammatory cytokine and chemokine gene expression through the opening of ORAI1 Ca2+ channels in the surface membrane of various immune cell-types within the lung. ORAI1 participates in a signalosome with the scaffolding protein A-kinase anchoring protein (AKAP)79/150 (encoded by the Akap5 gene), which binds numerous protein kinases, calcineurin and transcription factors required for cytokine expression. However, the role of AKAP79/150 in immune cell activation and the development of airway inflammation in response to allergens has not been established. We found that AKAP79/150 is expressed in mast cells and macrophages, where it has a pivotal role in driving pro-inflammatory cytokine production in response to house dust mite challenge. By contrast, AKAP79/150 expression in T cells was low and had little impact on cytokine release following T cell activation. Akap5-/- mice exhibited significantly reduced type 2 inflammation in the lungs following HDM exposure compared with wild-type controls. Our data demonstrate that AKAP79/150 is an important co-ordinator of inflammation within the lung in response to airborne allergens, through actions primarily on cells of the innate immune system.

PMID:
42406837
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

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