Protocol for generating and culturing high-grade serous ovarian carcinoma organoids from fresh or cryopreserved patient samples.

Authors

Laura Gall-Mas, Lidia Moyano-Galceran, Elina Valkonen, Johanna Hynninen, Krister Wennerberg, Wojciech Senkowski

Published in

STAR protocols. Volume 7. Issue 3. Pages 104690. Jul 06, 2026. Epub Jul 06, 2026.

Abstract

Stable long-term high-grade serous ovarian carcinoma (HGSC) organoids can be difficult to develop due to limited primary sample growth in 3D conditions. Here, we present a detailed protocol for the development of stable HGSC organoids, including all steps from seeding primary cells through culture monitoring, passaging, and expansion to viable cryopreservation and resuscitation. We provide guidance on initial passaging, passaging ratios, and criteria for successful culture establishment. Moreover, we include a troubleshooting section tackling recurrent problems in HGSC organoid development. For complete details on the use and execution of this protocol, please refer to Senkowski et al.¹.

PMID:
42406596
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

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