Structure-guided design of adsorbent: Online SPE-semi-preparative HPLC for efficient isolation and purification of three praeruptorins from Peucedanum Praeruptorum Dunn.

Authors

Xinli Yang, Zeyang Li, Xiaoya Jiang, Yanan Yuan, Huaizhong Guo, Ligai Bai

Published in

Journal of pharmaceutical and biomedical analysis. Volume 281. Pages 117642. Jul 04, 2026. Epub Jul 04, 2026.

Abstract

A novel ester-functionalized porous polymer adsorbent with a specific surface area of 268.5 m²/g was rationally synthesized via a one-step strategy, which enables selective adsorption of target coumarins relying on multiple intermolecular interactions (hydrogen bonding, hydrophobic effect, π-π stacking, etc.). An integrated online solid-phase extraction-semi-preparative HPLC system was established by coupling this tailor-made adsorbent with semi-preparative HPLC, realizing automated one-step extraction, enrichment and purification of target compounds directly from crude extracts. The loading volume and washing procedures were systematically investigated, and the optimal conditions were determined as follows: a sample loading volume of 3 mL, followed by washing with 0.1% aqueous phosphoric acid at a flow rate of 4.7 mL/min for 2 min. The integrated system exhibited excellent separation performance: praeruptorin A was obtained with 98% purity and a yield of 6.69 mg/g herb, and satisfactory isolation efficiency was achieved for praeruptorin B and praeruptorin C. Impressively, the adsorbent remained stable adsorption performance after 100 reuse cycles, significantly improving the cost-effectiveness of the purification process. This work provides a highly efficient and selective strategy for purifying bioactive coumarins from Peucedanum praeruptorum Dunn, and offers a versatile methodological blueprint and functional adsorbent platform for targeted isolation of high-value components from complex natural matrices, which will promote the research and development of natural product-based drug discovery and herbal medicine.

PMID:
42407148
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

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