Nuclear mechanotransduction: tools for mechanical perturbation and chromatin characterization.

Authors

Jennifer Soto, Nolan Origer, Yifan Wu, Braulio Cardenas Benitez, Ramzi Massad, Donna Rahgoshay, Abraham Lee, Timothy Downing, Song Li

Published in

Nucleus (Austin, Tex.). Volume 17. Issue 1. Pages 2690847. Dec 31, 2026. Epub Jul 06, 2026.

Abstract

Mechanical cues, ranging from matrix mechanical properties to dynamic mechanical loading, can be transmitted via structural proteins and signaling molecules to the nucleus to reorganize nuclear architecture and modulate chromatin accessibility. This mechanical regulation plays an important role in tissue regeneration and disease development. To gain deeper insights into the mechanical regulation of chromatin organization, it is essential to develop technologies that can apply mechanical inputs and characterize the resulting changes in nuclear structure and chromatin organization. Here, we review multidisciplinary technologies and tools that enable mechanical perturbation of the nucleus and the characterization of nuclear and chromatin responses. We highlight how perturbations such as matrix topography, confinement, stiffness, viscoelasticity, and dynamic loading can be used to apply mechanical cues to cells. We also discuss how imaging-based techniques, sequencing platforms, and computational approaches can be integrated to characterize nuclear architecture and chromatin organization in response to these mechanical stimuli.

PMID:
42411057
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.