Engineering a nanocluster-immobilized MOF@MOF architecture-based ratiometric fluorescence bioassay for sensing neurodegenerative disease-related acetylcholinesterase and inhibitor drugs.

Authors

Chao Liu, Jiadi Li, Fengyun Wang, Mengke Wang

Published in

Analytical methods : advancing methods and applications. Jul 07, 2026. Epub Jul 07, 2026.

Abstract

Accurate detection of acetylcholinesterase (AChE) activity and screening of its inhibitor drugs are crucial for detecting and intervening in neurodegenerative or aging-associated diseases. Herein, a sensitive and reliable ratiometric fluorescence platform is developed for sensing AChE activity and its inhibitor drugs. This strategy is based on an AZM hybrid, which is constructed by self-assembling a MOF@MOF architecture (ZIF8@MIL101(Al)) with AgAu nanoclusters (AgAuNCs) via electrostatic interactions. ZIF8 serves to enhance the fluorescence of AgAuNCs, while MIL101(Al) acts as a stable reference probe. Upon introduction of AChE and the substrate acetylthiocholine (ATCh), the generated thiocholine (TCh) quenches the AgAuNC fluorescence, whereas the MIL101(Al) signal stays constant, leading to a concentration-dependent change in the fluorescence intensity ratio. The ratiometric response enables sensitive detection of AChE activity with a limit of detection (LOD) of 0.032 U L^-1. The platform is applied to AChE determination in human serum samples. The system demonstrates the feasibility of inhibitor screening with tacrine as a model inhibitor. This work provides a simple ratiometric platform that holds promise for AChE-related clinical diagnosis and drug discovery.

PMID:
42411317
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

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