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DNA Nanobridge with Cascade Operation Logic on a Transistor for Comprehensive Evaluation of Respiratory Infection.

Created on 07 Jul 2026

Authors

Xiangyu Lv, Yanan Tang, Yang Yue, Mengzhu Wang, Chang Chen, Zihao Chen, Chaomin Wu, Xuejun Wang, Dacheng Wei

Published in

ACS applied materials & interfaces. Jul 07, 2026. Epub Jul 07, 2026.

Abstract

Acute respiratory infections may cause a cytokine storm that poses a great threat to life. Thus, on-site pathogen screening and infection severity assessment are highly desired for immediate medical intervention. Here, we report the comprehensive monitoring of respiratory infection by a graphene field-effect transistor (g-FET) modified with a DNA nanobridge that consists of two DNA tetrahedrons linked by a bridge strand. The recognition of target viral nucleic acid triggers bridge dissociation, releasing the probe to detect the inflammatory cytokine IL-6. Such a cascade logical operation enables simultaneous detection of respiratory pathogens and evaluation of the associated inflammatory response. The device detects viral nucleic acid as low as 3.9 × 10-16 M, while the presence of IL-6 at a clinically relevant level of 2.4 × 10-12 M enhances the response by 3.7-fold. Thus, testing of clinical COVID-19 oropharyngeal swabs achieves ∼97.2% overall agreement with PCR results, demonstrating immense potential in practical applications.

PMID:
42411119
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.

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