Authors
Chenglong Li, Xiaonan Zhou, Yanling Ding, Junjie Xu, Jiahao Liang, Li'e Hou, Xiaolong Kang
Published in
Animal genetics. Volume 57. Issue 4. Pages e70163.
Abstract
Intramuscular fat (IMF) content is a critical determinant of meat quality, directly influencing flavor and palatability. RNA editing, a prevalent posttranscriptional modification in mammals, has been extensively investigated in cattle; however, its role in bovine intramuscular fat deposition remains poorly understood. Here, we performed a comprehensive analysis of RNA editing differences in the longissimus dorsi muscle between cattle groups with divergent IMF content to identify editing sites potentially associated with IMF deposition. A total of 283 581 RNA editing sites (RESs) were identified from transcriptomic data of cattle with contrasting IMF phenotypes. Among the differential A-to-I editing sites located in coding sequences, 14 led to amino acid alterations, including an editing event in MYBPH, a gene with important functions in muscle and adipose tissues. Differential A-to-I editing sites in the 3' UTR resulted in altered miRNA binding patterns: 30 sites led to loss of miRNA binding, whereas 16 sites gained novel miRNA binding capacity. Dual-luciferase reporter assays confirmed that the editing site chr2:106787199 in the 3' UTR of PRKAG3 affects binding by bta-miR-23b-5p. Functional validation experiments demonstrated that bta-miR-23b-5p promotes PRKAG3 expression, and overexpression of PRKAG3 suppresses preadipocyte differentiation and lipid droplet formation. Collectively, these findings uncover a regulatory role for RESs in IMF deposition and provide new insights into the functional significance of RNA editing in bovine intramuscular fat accumulation.
PMID:
42412410
Bibliographic data and abstract were imported from PubMed on 07 Jul 2026.
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