Jagged1⁺ Tregs mediate lymphatic remodeling in tumor-draining lymph nodes.

Authors

Jessie Z Xu, Pei-Hsun Tsai, Prudence PokWai Lui, Jie Ting Wang, Hafsah Aziz, Boyu Xie, Inchul Cho, Manuela Terranova-Barberio, Chao Zheng Li, Katie Stoker, Hongqiang Yu, Alice E Denton, Toby Lawrence, Sophia N Karagiannis, Niwa Ali

Published in

Cell reports. Volume 45. Issue 7. Pages 117597. Jul 06, 2026. Epub Jul 06, 2026.

Abstract

Tumor-draining lymph nodes (tdLNs) undergo extensive stromal remodeling during tumor progression, but the immune signals that shape this process remain unclear. Here, using the B16-F10 melanoma model, we examine the role of Jagged1-expressing regulatory T cells (Jag1⁺ Tregs) in tdLN remodeling. Jag1 expression is increased in late-stage versus early-stage tdLN Tregs. Conditional deletion of Jag1 in Tregs (Foxp3^ΔJag1) attenuates tdLN expansion without altering effector T cell abundance or activation. Bulk transcriptomic analysis identifies stromal-related transcriptional changes in Foxp3^ΔJag1 tdLNs, including altered expression of genes linked to lymphatic endothelial cell (LEC) biology. Consistent with this, flow cytometry and histological analysis show reduced tdLN lymphatic expansion, while fluorescein isothiocyanate (FITC)-dextran tracing indicates altered lymphatic drainage. Jag1⁺ Tregs are also detected in tdLNs in patients with melanoma. Together, these findings identify Jag1⁺ Tregs as regulators of the tdLN lymphatic microenvironment during melanoma progression.

PMID:
42412612
Bibliographic data and abstract were imported from PubMed on 08 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.