Protocol for minimized-bias profiling of liver and visceral adipose tissue in mice using integrated single-nucleus RNA sequencing.

Authors

Lei Li, Jonathan G Pol, Christophe Desterke, Wanchao Hu, Nicolas Trainel, Morgane Benardeau, Manuela Lizarralde-Guerrero, Maria Chiara Maiuri, Guido Kroemer, Gabriel Perlemuter, Anne-Marie Cassard, Cosmin Sebastian Voican

Published in

STAR protocols. Volume 7. Issue 3. Pages 104699. Jul 08, 2026. Epub Jul 08, 2026.

Abstract

Single-nucleus RNA sequencing (snRNA-seq) enables transcriptomic profiling of tissues that are difficult to dissociate. Here, we present an integrated protocol combining tissue-specific nuclei extraction and selective enrichment to isolate high-quality nuclei from fresh mouse liver and visceral adipose tissue (VAT). We describe steps for single-nucleus extraction, magnetic enrichment, BD Rhapsody capture, cDNA synthesis, whole-transcriptome amplification, index PCR, library quality control, and shallow sequencing-based assessment of cell-type recovery, while separating whole-liver and non-parenchymal cell workflows to reduce dissociation bias.

PMID:
42418327
Bibliographic data and abstract were imported from PubMed on 09 Jul 2026.

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