Single-cell transcriptomics and DNA methylomics reveal cell-type specific heat stress response mechanisms in alfalfa.

Authors

Yanmin Hu, Jinsong Zhang, Yao Liu, Zhujiang Liu, Yuning Hu, Fengye Xu, Jie Liu, Xianjun Peng

Published in

Plant physiology. Jul 09, 2026. Epub Jul 09, 2026.

Abstract

Heat stress severely limits crop yield and threatens global food security, also significantly influence the growth and development of alfalfa. Although there have been some studies on heat stress at the organ or tissue levels, the heat stress response mechanisms on the cell type level still remains unclear in alfalfa. Here, the single-nucleus RNA sequencing (snRNA-seq) was performed on 61,835 high-quality leaf cells under control and heat-stressed conditions (1 h and 48 h), revealing distinct transcriptional responses across five major cell types identified from 17 cell clusters. A total of 5,098 differentially expressed genes (DEGs) were detected, and cell-type-specific regulators involving lignin biosynthesis (PAL, 4CL, OMT, CCoAOMT), hormone signaling (Aux, IAA, ARP, PIN, ERF, EIN, ASR), and kinase cascades (LRR-RLK, PP2C, WAK, and STPK) in vascular, epidermal, and mesophyll cells were uncovered by pseudo-time trajectory analyses, respectively. The weighted gene co-expression network analysis (WGCNA) identified ten cell-type-specific heat-responsive networks, and some key heat-responsive genes (HSF, HSP, MAPK, CML, CIPK, and WRKY) exhibited opposing expression patterns in different cell types, highlighting the complexity regulatory mechanisms across cell types under heat stress. Furthermore, the whole-genome bisulfite sequencing (WGBS) identified 6,252 differentially methylated regions and revealed a global increase in DNA methylation under heat stress. By integrating snRNA-seq, WGBS, and pseudo-time trajectory analyses, MsHSP90 and MsHSFA5 were core transcription factors appearing repeatedly. Functional studies demonstrated that MsHSP90 and MsHSFA5 significantly influence plant thermotolerance. Overall, our study provided valuable insights into the heat stress response mechanisms of alfalfa at the single-cell level.

PMID:
42424531
Bibliographic data and abstract were imported from PubMed on 10 Jul 2026.

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