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Assembly of Silphium interspecific hybrid genomes opens the genus to phylogenomics, ecogenomics, and molecular breeding.

Created on 10 Jul 2026

Authors

Renan S Souza, Josh P Clevenger, Jerry Jenkins, Walid Korani, Jeremy Schmutz, Jane Grimwood, Christopher Plott, Yaniv Brandvain, Kathryn Turner, Allison J Miller, Brent S Hulke, Louis F Nastasi, Ebony G Murrell, Lori H Handley, Jenell Webber, Lori Beth Boston, Stella Woeltjen, Matthew J Rubin, Betsy Trana, Erin Howard, Ashlyn Bazzel, Alex Harkess, David L Van Tassel

Published in

Nature communications. Jul 09, 2026. Epub Jul 09, 2026.

Abstract

Wild perennial plants can be domesticated to make agriculture more diverse and resilient, but many have large genomes that have been recalcitrant to analysis. Here, we report phased genome assemblies for Silphium integrifolium Michx. and S. perfoliatum L., two species native to North America under domestication, and demonstrate the utility of trio-binning for genome assembly using an interspecific hybrid. These genomes have chromosomes reaching 1.8 Gb and a helical structure preserved during interphase with a loop circumference of 43 Mb. A genome-informed low coverage and target sequencing strategy enables the refinement of the genus phylogeny, reveals the spatial distribution and structure of natural populations, and identifies 81 loci associated with environmental and domestication traits. Variants in a MATE transporter, α/β hydrolase, and ortholog of Arabidopsis ACT Domain Repeat (ACR4) protein explain significant variance in floral architecture. These advances in genome assembly and genotyping could expand the range of candidates for de novo crop domestication.

PMID:
42426026
Bibliographic data and abstract were imported from PubMed on 10 Jul 2026.

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