A simplified and high-yield purification method for recombinant RNase R.

Authors

Wataru Horikawa, Daniel L Kiss

Published in

microPublication biology. Volume 2026. Epub Jun 24, 2026.

Abstract

RNase R is a 3'→5' exoribonuclease that selectively degrades linear RNAs. As such, RNase R has become a key reagent for circRNA production and analysis. This is particularly true for the in vitro production of circRNA research tools and therapeutic candidates. Unfortunately, the high cost of commercial RNase R is restrictive. We report an accessible, cost-effective method to purify high-quality recombinant RNase R from E. coli using single-step Ni NTA chromatography on entry level FPLC systems. By achieving complete linear RNA digestion while maintaining circRNA, the resulting enzyme matches performance attributes of commercially available RNase R.

PMID:
42428298
Bibliographic data and abstract were imported from PubMed on 10 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.