Kinetics of anti-SARS-CoV-2 IgG in saliva and serum post infection, and subsequent vaccination in South Africa.

Authors

Maemu P Gededzha, Mixo Sibiya, Celine Pellaton, Kubashni Woeber, Duduzile Nsibande, Nobuhle Mchunu, Brodie Daniels, Terusha Chetty, Reshmi Dassaye, Khanya Mohlabi, Shameem Jaumdally, Keertan Dheda, Ruth Lekalakala, Shabir A Madhi, Elizabeth Mayne, Glenda Gray, Yves Levy, Craig Fenwick, Song Ding, Penny L Moore, Ameena Goga

Published in

African journal of laboratory medicine. Volume 15. Issue 1. Pages 3052. Epub Jun 26, 2026.

Abstract

Understanding mucosal immune response to Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) infection and vaccination, and its correlation to systemic responses could improve vaccine development. There is limited data on mucosal and systemic antibody responses to SARS-CoV-2 infection and vaccination in African populations.
This study assessed serum and saliva antibody ratios in a cohort of SARS-CoV-2 infected and vaccinated participants followed up for 12 months.
This longitudinal study comprised of 67 SARS-CoV-2, PCR-confirmed participants who were recruited as part of a COVID-19 Point of Care Study, between January 2021 and September 2022. For this analysis, 254 serum and 214 saliva samples (205 of which were matched), were collected at baseline, 6, 9, and 12 months. Antibodies against Spike (S) and nucleocapsid (N) SARS-CoV-2 were assessed by in-house Luminex assay.
Serum and saliva anti-S IgG from were detected for up to 12 months in majority of participants (93.5% and 90%), regardless of vaccination status. There was a concordance between paired serum and saliva antibody in 80.5% anti-S IgG and 41.7% anti-N IgG of samples. There was a weak but significant correlation between the matched serum and saliva samples (ρ = 0.42, p < 0.001 for anti-S and ρ = 0.33, p < 0.001 for anti-N). Longitudinal analysis in participants who were vaccinated with either the Ad26.COV2 or BNT162b2 COVID-19 vaccine after their baseline visit, serum anti-S IgG significantly increased (p = 0.023 and p = 0.038), compared with unvaccinated participants. Breakthrough infections or reinfections were identified in (35/62) 56.5% of vaccinated and unvaccinated participants.
This study reports the correlation of the anti-S and anti-N IgG responses in serum and saliva following SARS-CoV-2 infection and subsequent vaccination in low-middle income setting. Furthermore, high breakthroughs and reinfection were reported.
The study findings are consistent with those from high-income settings, supporting correlation between systemic and mucosal immunity and booster vaccination as a long-term strategy to control SARS-CoV-2.

PMID:
42428982
Bibliographic data and abstract were imported from PubMed on 10 Jul 2026.

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