Authors
Anna Zdubek, Irena Maliszewska, Rafał Kowalczyk, Bartosz Turek, Renata Grzywa
Published in
Bioorganic chemistry. Volume 180. Pages 110202. Jul 03, 2026. Epub Jul 03, 2026.
Abstract
This study investigates the enhanced efficacy of photoinduced microbial inactivation targeting both planktonic and biofilm forms of Gram-negative bacteria, specifically Acinetobacter baumannii and Proteus mirabilis. The approach utilizes diaminocyclohexane (DACH)-based diamines to augment antibacterial photodynamic therapy (aPDT) employing 5-aminolevulinic acid. Results demonstrated that blue light irradiation produced the highest bactericidal effects, achieving mortality rates of 99.9990 ± 0.0008% for P. mirabilis and 99.990 ± 0.001% for A. baumannii at a light dose of 70 J·cm-2 in the presence of a non-cytotoxic concentration of (1R,2R)-N,N'-bis([1,1'-biphenyl]-4-ylmethyl)cyclohexane-1,2-diamine. The biofilm destruction efficiency was suboptimal, with maximum bactericidal efficiencies of 87.1 ± 0.1% and 73.1 ± 0.2% for P. mirabilis and A. baumannii, respectively, after exposure to blue light at a dose of 93.5 J·cm-2. Increased accumulation of protoporphyrin IX within bacterial cells was identified as a key factor contributing to the enhanced photobiocidal efficacy. Additionally, molecular docking analysis was employed to explore potential structural insights into the biological activity of this cyclohexane-1,2-diamine-based ligand. Computational simulations suggested that the ligand could plausibly accommodate within the protoporphyrin IX binding pocket of the modeled ferrochelatase, indicating a potential binding mode compatible with the catalytic cavity.
PMID:
42430819
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.
Read full publication at:
Please sign in
to see all details.
Advertisement
Stats
- Recommendations n/a n/a positive of 0 vote(s)
- Views 1
- Comments 0