Phelligrin B, a flavonoid compound from Sanghuangporus vaninii, improves CCl₄-induced acute liver injury associated with the PI3K-AKT1-mTOR pathway.

Authors

Xiaodong Wei, Jialu Qiao, Jingjing Wang, Mingxing Zhang, Zhaofeng Wang, Yanping Zeng

Published in

Bioorganic chemistry. Volume 180. Pages 110231. Jul 07, 2026. Epub Jul 07, 2026.

Abstract

Acute liver injury (ALI) is a severe condition with high risk of progression to liver failure. Traditional Chinese Medicine plays an important role in liver protection. This study aimed to investigate the protective effects and mechanism of Sanghuangporus vaninii against ALI. Network pharmacology identified active components and their potential targets. Molecular docking and dynamics simulations validated the binding interactions between active components and key targets. In vivo CCl₄-induced ALI mice were pretreated with Phelligrin B to assess liver pathology, function, oxidative stress, inflammation, and PI3K-AKT1-mTOR pathway expression. In vitro CCl₄-injured hepatocytes were treated with Phelligrin B to evaluate viability, LDH release, oxidative stress, inflammation, and the PI3K-AKT1-mTOR pathway activation. Results showed that 15 active flavonoids were identified from Sanghuangporus vaninii, sharing 137 common targets with ALI, among which AKT1 was the most significant. Molecular docking analysis of the main active components with AKT1 revealed Phelligrin B as the compound with the highest binding affinity. Further molecular dynamics simulations confirmed a stable binding mode. In vivo studies demonstrated that Phelligrin B significantly alleviated liver injury, oxidative stress, and inflammation in the ALI mouse model. And Phelligrin B notably suppressed AKT1 protein expression and inhibited the activation of the PI3K-AKT1-mTOR pathway. Similarly, in vitro, Phelligrin B counteracted CCl₄-induced reductions in cell viability and increases in LDH release, mitigated oxidative stress and inflammatory responses, and suppressed the activation of the PI3K-AKT1-mTOR pathway in the cellular model. In conclusion, Phelligrin B, the flavonoid (specifically, a dihydroflavone derivative) from Sanghuangporus vaninii alleviates CCl₄-induced ALI, an effect associated with inhibition of the PI3K-AKT1-mTOR pathway.

PMID:
42430818
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.

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