Authors
Gilles Dupouy, Tamsin Spelman, Gaurav Singh, Etienne Herzog, Stephanie Baudrey, Simone Bovio, Jérome Mutterer, Olivier Hamant, Alexandre Berr, Henrik Jönsson, Marie-Edith Chabouté
Published in
The Plant cell. Volume 38. Issue 7. Jul 02, 2026.
Abstract
In plants, polar cell growth is essential for processes such as root hair and pollen tube growth, but how it integrates with nuclear movement, cytoskeletal organization, and cell mechanics is not fully understood. We conducted high-resolution live imaging of Arabidopsis thaliana root hairs throughout their development using a microfluidic device. We identified 3 distinct stages--fast growth, slow growth, and early maturation--and quantified growth kinetics at high temporal resolution. The transition from fast to slow growth was consistent with cytoskeletal dynamics causing reduced tip growth and decreased nucleus-tip distance. Based on these observations, we developed a mathematical model linking cytoskeletal dynamics with tip growth and nuclear dynamics. Through genetic and pharmacological approaches, we were able to disrupt or trigger this transition, supporting the model and revealing the existence of essential crosstalk between actin filaments and microtubules. Additionally, vacuole dynamics, root hair diameter, and cell stiffness changed during the fast-to-slow transition, indicating a coordinated regulation of multiple subcellular systems. Together, these results connect nuclear, cytoskeletal, and mechanical dynamics during root hair development, offering an integrated view of the subcellular processes behind the control of polar cell growth in plants.
PMID:
42430653
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.
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