Authors
Hannah K Jaeger, Michael Denton, Takeshi F Andoh, Craig N Kreklywich, Lina Gao, Lydia J Pung, Zachary J Streblow, Ann McMonigal, Karina Ray, Brayden Graves, Magdalene M Streblow, Aaron M Barber-Axthelm, Gavin Zilverberg, Margaret Terry, Suzanne S Fei, Glenn Hogan, David C Schultz, Sara Cherry, Michael K Axthelm, Caralyn S Labriola, Mark T Heise, Daniel N Streblow
Published in
PLoS neglected tropical diseases. Volume 20. Issue 7. Pages e0013780. Jul 10, 2026. Epub Jul 10, 2026.
Abstract
O'nyong-nyong virus (ONNV) is a mosquito-borne alphavirus first isolated in Uganda in 1959. Since its discovery, ONNV has caused several outbreaks in Africa, manifesting clinically as fever, rash, and joint/muscle pain lasting months. Currently, we have a limited understanding of ONNV infection and disease in relevant animal models, which restricts the evaluation of vaccines and therapeutics. In 1967, Binn et al. reported that infection of rhesus macaques (RMs) with ONNV failed to induce viremia in two animals. This may be attributed to the potential attenuation of the virus through extensive passaging. To mitigate this issue, we constructed an infectious clone from the sequence of ONNV-UVRI0804 (ONNV0804), a 2017 clinical isolate from a febrile patient in Uganda. This strain demonstrated high pathogenicity in immunocompetent mice, resulting in an earlier and more severe onset of disease and significantly higher viremia compared to a highly passaged control strain ONNVUgMP30. In the current study, three male and three female rhesus macaques were subcutaneously inoculated with ONNV0804. All animals became viremic at 2 days post inoculation (dpi). Both classical and nonclassical monocytes were activated (CD169+), peaking at 3 dpi, which corresponded with the peak of plasma viral RNA. Additionally, CD4+ and CD8 + effector memory T cells and memory B cells began proliferating in peripheral blood by day 7, peaking at day 10, which also corresponded to the timing of neutralizing antibody development, indicating a robust adaptive immune response to ONNV0804. Finally, key clinical disease manifestations were recapitulated, including lymphadenopathy and histological features of early-stage arthritis. Taken together, rhesus macaque infection with ONNV0804 clinical isolate is a promising model for investigating immune responses to alphaviruses and evaluating vaccines to protect against future epidemics.
PMID:
42430456
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.
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