Protocol for evaluating microglial phagocytosis in situ in mouse hippocampal slices.

Authors

Summer G Paulson, Fritz W Lischka, Jeremy D Rotty

Published in

STAR protocols. Volume 7. Issue 3. Pages 104696. Jul 10, 2026. Epub Jul 10, 2026.

Abstract

Microglia are the primary phagocytes of the central nervous system (CNS). Here, we present a protocol for microinjecting opsonized particles into mouse hippocampal slices followed by two-photon time-lapse microscopy. We describe steps for preparing components, removing the brain, creating coronal slices, and incubating samples. We then detail procedures for analyzing microglial morphology and cell process dynamics with microinjected particles. We also provide several common potential problems encountered during the protocol alongside troubleshooting strategies to counter them. For complete details on the use and execution of this protocol, please refer to Paulson et al.¹.

PMID:
42430244
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.