Protocol for assessing enzymatic and non-enzymatic hydrogen sulfide production and levels.

Authors

María Ángeles Cáliz-Molina, Inmaculada Pino-Pérez, András K Ponti, Christopher Hine, Alejandro Martín-Montalvo

Published in

STAR protocols. Volume 7. Issue 3. Pages 104694. Jul 09, 2026. Epub Jul 09, 2026.

Abstract

Hydrogen sulfide (H₂S) is a key molecule in metabolism with important physiological effects. Despite its merits, detection and/or accurate determination remain challenging. Here, we present a protocol to determine H₂S levels and cumulative production using a microsulfide ion electrode and lead acetate paper. We describe steps for measurement of H₂S levels, as well as detection of enzymatic and non-enzymatic cumulative H₂S production across various biological contexts. This protocol is thus compatible with H₂S donors, tissue samples, and live cultured cells. For complete details on the use and execution of this protocol, please refer to Caliz-Molina et al.¹.

PMID:
42430243
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.

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