Halorubrum marinum sp. nov., Halorubrum rarum sp. nov., Halorubrum wangae sp. nov., Halorubrum shenae sp. nov., and Halorubrum zhoui sp. nov., halophilic archaea from coastal tidal flats, a saline lake, and a marine solar saltern.

Authors

Qing-Di Li, Yang-Yang Wang, Chidiebele Nwankwo, Yao Hu, Xin-Yue Dong, Jing Hou, Xiang-Dong Chen, Heng-Lin Cui

Published in

Systematic and applied microbiology. Volume 49. Issue 5. Pages 126750. Jul 08, 2026. Epub Jul 08, 2026.

Abstract

Five novel halophilic archaeal strains, designated DTA46^T, DTA98^T, HHNYT27^T, N11^T, and SY-15^T, were isolated from diverse saline environments across various regions of China. Amplicon and metagenome analyses revealed that three amplicon reads were affiliated with strains DTA46^T, HHNYT27^T, and N11^T while two MAGs related to strains N11^T and SY-15^T. The sequence similarities among these five strains and current species of the genus Halorubrum were 93.1%-99.1% and 86.0%-95.9% judged by 16S rRNA and rpoB' genes, respectively. Phylogenomic and comparative genomic analyses revealed their close affiliation with Halorubrum. The average nucleotide identity (ANI), digital DNA-DNA hybridization (dDDH), and average amino acid identity (AAI) values between these strains and existing Halorubrum species ranged from 74.9%-93.6%, 22.3%-58.3%, and 68.3%-93.7%, respectively. All are below the recommended thresholds for species delineation, which supports their classification as novel taxa. The growth characteristics of strains DTA46^T, DTA98^T, HHNYT27^T, N11^T, and SY-15^T were determined as follows: temperature range 20-60 °C (optima: 35, 37-42, 37, 35, and 42 °C), NaCl concentration 1.4-5.5 M (optima: 2.6, 3.1, 3.1, 3.1, and 5.1 M), and pH range 5.5-9.5 (optima: 8.0, 8.0, 7.0, 7.5, and 7.0). Based on the polyphasic characterization integrating phenotypic, chemotaxonomic, phylogenetic, and phylogenomic evidence, strains DTA46^T, DTA98^T, HHNYT27^T, N11^T, and SY-15^T are proposed to represent five novel species of the genus Halorubrum, for which the names Halorubrum marinum sp. nov., Halorubrum rarum sp. nov., Halorubrum wangae sp. nov., Halorubrum shenae sp. nov., and Halorubrum zhoui sp. nov. are designated, respectively.

PMID:
42430840
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.

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