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Recent advances in bioconjugation via CuAAC click reaction: Synthesis and bio-applications.

Created on 11 Jul 2026

Authors

Anurag Shukla, Yogesh Yadav, Ram Sagar

Published in

Bioorganic chemistry. Volume 180. Pages 110229. Jul 06, 2026. Epub Jul 06, 2026.

Abstract

Click chemistry is a highly reliable and straightforward method for bioconjugation. It exhibits high selectivity and biocompatibility, rendering biorthogonal tagging and regenerative medicine. Its transformative applications in targeted therapy, drug delivery, molecular imaging, and nanotechnology are paving the way for groundbreaking advancements in chemistry and biology. In this review, we discuss the detailed application of Copper-catalysed azide-alkyne cycloaddition (CuAAC), with limited examples of SPAAC, IEDDA, SuFEX, SeNEx and PFEx including recent advances in bioconjugation through the application of bioorthognal methods using click chemistry, focusing on the synthesis of privileged bioconjugates during 2020-2025. Thus, this review highlights the significance of click chemistry as a powerful method for bioconjugation, providing a valuable resource for the scientific community on click chemistry and its applications in biomedical sciences.

PMID:
42430826
Bibliographic data and abstract were imported from PubMed on 11 Jul 2026.

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