Generation of apical-out nasal organoids to facilitate human respiratory syncytial virus infection and drug screening.

Authors

Georgios Stroulios, Mathieu Hubert, Wing Chang, Allen Eaves, Sharon Louis, Philipp Kramer, Caroline Tapparel, Salvatore Simmini

Published in

iScience. Volume 29. Issue 7. Pages 116638. Jul 17, 2026. Epub Jul 02, 2026.

Abstract

The nasal epithelium is the first respiratory epithelium that is exposed to inhaled airborne pathogens. As a result, it is crucial to model host-pathogen interactions occurring in this tissue. To facilitate the efficient modeling of these interactions, we have developed a method to generate de novo apical-out nasal organoids from nasal epithelial cell aggregates. Optimization of this method revealed a stark tissue-specific effect of the culture temperature, as organoids were generated in much higher efficiency at 32.5°C compared to more widely used temperatures of 37°C. These organoids recapitulate the native tissue cellular composition of ciliated, basal, and goblet cells, while maintaining high homogeneity in size. Functionally, the system demonstrates susceptibility to viral infection and provides a robust platform for modeling antiviral drug responses. This standardized approach offers a reproducible system with high potential to be utilized in host-pathogen interaction studies and personalized medicine.

PMID:
42437002
Bibliographic data and abstract were imported from PubMed on 12 Jul 2026.

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