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The impact of alcohol-based and chlorhexidine-based disinfectants on hand microorganisms, skin tolerance and nosocomial infections among healthcare workers.

Created on 12 Jul 2026

Authors

Yuan Xu, Haiyan Chen, Wen Li

Published in

Pakistan journal of pharmaceutical sciences. Volume 39. Issue 9. Pages 2837-2852.

Abstract

Hand hygiene is critical for preventing hospital-acquired infections (HAIs), yet the comparative effects of alcohol-based versus chlorhexidine-based disinfectants on microbial load, skin tolerance and infection rates among healthcare workers (HCWs) remain underexplored within the same clinical setting.
To compare the impact of alcohol-based and chlorhexidine-based disinfectants on hand microbiota reduction, skin tolerance and the incidence of HAIs among HCWs.
This retrospective cohort study analyzed 136 healthcare workers at a single hospital. Participants were divided into two phases: an alcohol-based disinfectant phase from January 1, 2022, to June 30, 2022 and a chlorhexidine-based disinfectant phase from July 1, 2022, to December 31, 2022. Bacterial counts pre- and post-disinfection, skin condition assessments and hospital acquired infection (HAI) rates were analyzed.
Immediate bactericidal efficacy was superior in the alcohol group (1.60 ± 0.15 CFU/cm²) compared to the chlorhexidine group (2.80 ± 0.61 CFU/cm², P < 0.001). Chlorhexidine exhibited more sustained antimicrobial effects, with superior efficacy at 4 hours post-disinfection (7.20 ± 1.32 CFU/cm² vs. 9.53 ± 1.66 CFU/cm² for alcohol, P < 0.001). Skin tolerance was better for chlorhexidine. HAI incidence was slightly lower in the chlorhexidine group (3.80%) compared to the alcohol group (5.76%, P = 0.427).
Alcohol provides immediate bactericidal action and chlorhexidine offers prolonged effects. It is recommended to use disinfectants according to the preferences of healthcare personnel.

PMID:
42437330
Bibliographic data and abstract were imported from PubMed on 12 Jul 2026.

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