The peptidoglycan endopeptidase MepH of uropathogenic Escherichia coli supports competitive fitness during prolonged urinary tract infections.

Authors

Wen-Chun Huang, Masayuki Hashimoto, Jiun-Ling Wang, Jiunn-Jong Wu, Ming-Cheng Wang, Cheng-Yen Kao, Wei-Hung Lin, Ching-Hao Teng

Published in

Scientific reports. Jul 12, 2026. Epub Jul 12, 2026.

Abstract

Urinary tract infections (UTIs) are predominantly caused by uropathogenic Escherichia coli (UPEC) and are characterized by frequent recurrence and persistence despite antibiotic treatment. Successful infection requires dynamic remodeling of the bacterial envelope to withstand host-derived stresses. Peptidoglycan endopeptidases mediate cell wall remodeling, yet the contribution of MepH to UPEC pathogenesis remains unclear. Here, we investigated the role of MepH using the prototypical UPEC strain UTI89. In a murine UTI model, deletion of mepH significantly reduced bacterial fitness in the urine at 2 days post-infection, as well as in the urine, bladder, and kidneys at 14 days post-infection. The mepH mutant exhibited impaired survival in mouse urine in a manner dependent on heat-labile components, decreased filamentous morphological switching in vivo, increased susceptibility to macrophage-mediated killing, and reduced resistance to detergent stress, while motility, epithelial cell association, serum resistance, and acid tolerance were unchanged. These findings identify MepH as a previously unrecognized virulence determinant that promotes prolonged UPEC infection, highlighting peptidoglycan remodeling enzymes as potential targets for anti-virulence strategies.

PMID:
42437800
Bibliographic data and abstract were imported from PubMed on 13 Jul 2026.

Read full publication at:
Please sign in to see all details.

Stats

1-terrible, 9-excellent. How would you rate this publication? Sign in in to submit your rating.

Post a comment

You need to be signed in to post comments. You can sign in here.

Comments

There are no comments yet.