Authors
Dixhya Rani, Meenakshi Sharma, Urvashi Dhiman, Tashi Palmo, Ashutosh Shahi, Kuljit Singh, Prasoon Gupta
Published in
Medicinal chemistry research : an international journal for rapid communications on design and mechanisms of action of biologically active agents. Volume 35. Issue 6. Pages 1161-1185. Epub May 18, 2026.
Abstract
This study aimed to identify potent antimicrobial compounds from Ayurvedic plant Oroxylum indicum. Phytochemical examination of its stem bark led to the isolation of 29 chemical constituents, including five compounds for the first time from this genus: Chrysophanol (12), Ipriflavone (15), Pinoresinol diglucopyranoside (22), Quercetin 3-O-β-D-glucofuranoside (28), and 5-hydroxy-3,7,8,3',4'-pentamethoxyflavone (29). Notably, compound 28 was a unique flavonoid bearing a β-D-glucofuranoside moiety, very rare in this class. Structural elucidation of the compounds (1-29) was achieved using 1D/2D NMR and HRESI-MS spectrometry. Antimicrobial susceptibility analysis identified two potent compounds (28 and 29) exhibiting MIC and MBC in the range of 0.09 µM to 1.5 µM and 0.7 µM to 6.25 µM against the tested bacterial pathogens, respectively. Further, bactericidal potential of the two identified hit were validated by time-kill kinetics studies. Cytotoxicity assessment in mammalian macrophage cell lines (RAW 264.7 and J774A.1) and hemolysis assays represented an acceptable cytocompatibility profile of these two molecules within the tested concentration range. These findings highlight molecules (28 and 29) as promising compounds for the development of novel antibacterial against Gram-positive and Gram-negative pathogens.
PMID:
42446684
Bibliographic data and abstract were imported from PubMed on 14 Jul 2026.
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