Authors
Da Zheng, Huapeng Chen, Huan Liang, Jian Luo, Feibiao Song
Published in
Fish physiology and biochemistry. Volume 52. Issue 4. Jul 14, 2026. Epub Jul 14, 2026.
Abstract
This study investigated the effects of dietary 17α-methyltestosterone (MT) on gonadal differentiation and transcriptomic changes associated with partial masculinization in Trachinotus blochii. Larvae (3 days post-hatching) were fed a diet containing 100 mg/kg MT for 60 days. The results showed that MT treatment induced intersex gonadal development in 73% of genetic females, with gonads characterized by the presence of spermatogonia. Hormone assays showed increased MT levels and reduced 17β-estradiol (E2) levels in MT-treated intersex individuals compared with control females. Compared with control females, MT-treated intersex individuals showed increased expression of male-associated genes, including cyp11b1, sf1, and dmrt1, whereas cyp19a1a was reduced. However, other female-associated genes, such as hsd17β1 and foxl2, were not completely suppressed. Transcriptome profiling identified significant enrichment of FoxO-related and steroid hormone biosynthesis pathways. Trend analysis suggested that changes in oocyte meiosis-related and steroid hormone biosynthesis-related genes may be associated with MT-induced gonadal remodeling. These findings provide transcriptomic evidence for pathways potentially associated with MT-induced intersex gonadal development and may inform future studies on sex-control strategies in T. blochii, although fertility testing and reproductive validation of these intersex individuals are still required.
PMID:
42446810
Bibliographic data and abstract were imported from PubMed on 14 Jul 2026.
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