Co-regulation of HIV control and cytomegalovirus pp65-specific IL-1β and TNF-α responses by genetic variants in the MHC region.

Authors

Suzanne D E Ruijten, Jéssica C Dos Santos, Victoria Rios-Vazquez, Adriana Navas, Hanneke Maas, Albert L Groenendijk, Marc J T Blaauw, Louise E van Eekeren, Wilhelm A J W Vos, Rainer Knoll, Manoj Kumar Gupta, Rob Ter Horst, Javier Botey-Bataller, Nienke van Unen, Yang Li, Anna C Aschenbrenner, Joachim L Schultze, Cheng-Jian Xu, Mihai G Netea, Andre J A M van der Ven, Vasiliki Matzaraki

Published in

PLoS pathogens. Volume 22. Issue 7. Pages e1014355. Epub Jul 14, 2026.

Abstract

The spontaneous control of HIV infection in the absence of antiretroviral therapy, termed HIV control, is associated with genetic variation in the Major Histocompatibility Complex (MHC) locus. These variants are known to influence the immune response to HIV itself. However, people living with HIV are often co-infected with other pathogens that can also elicit immune responses, which might also be regulated by these variants. Here, we assessed whether genetic variants associated with HIV control influence cytokine responses to various co-pathogens. HIV-control-associated single nucleotide polymorphisms (SNPs) were enriched among variants regulating TNF-α and IL-1β production upon CMV pp65 peptide pool stimulation. The top enriched SNPs, rs1128175-A and rs2853971-A, were linked to lower odds of HIV control and increased cytokine responses to CMV. These SNPs were in linkage disequilibrium (LD) with classical HLA alleles HLA-B*07:02 and HLA-C*07:02. Intracellular cytokine staining showed CMV serostatus-dependent production of TNF-α by monocytes and CD8 T cells. The rs1128175-A/rs2853971-A/HLA-B*07:02/HLA-C*07:02 haplotype was associated with increased IFN-γ production by CD8 T cells upon CMV pp65 peptide pool stimulation, indicating an effect on memory responses. Quantitative trait locus (QTL) mapping showed that rs1128175 and rs2853971 influence HLA-B and HLA-C expression, DNA methylation levels and cell-type-specific cis-effects on chromatin accessibility, as well as CD8 T cell subset abundance. These QTL associations suggest that variants associated with poor HIV control are linked to heightened pro-inflammatory responses to CMV pp65 through effects on antigen presentation, epigenetic modifications, gene expression and immune cell repertoire, potentially negatively affecting HIV control status.

PMID:
42447072
Bibliographic data and abstract were imported from PubMed on 15 Jul 2026.

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