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Topical mosquito repellency of a Litsea cubeba essential oil spray against Aedes aegypti, Anopheles dirus, and Culex quinquefasciatus.

Created on 15 Jul 2026

Authors

Nataya Sutthanont, Jiraporn Leanpolchareanchai, Patchara Sriwichai, Jiraporn Ruangsittichai, Theerawit Phanpoowong, Suchada Sumruayphol, Thipruethai Phanitchat, Toshio Sakai, Yuki Eshita, Raweewan Srisawat

Published in

Scientific reports. Jul 14, 2026. Epub Jul 14, 2026.

Abstract

Mosquito-borne diseases remain an important global public health concern, and increasing insecticide resistance has strengthened interest in complementary personal protection tools. This study evaluated the short-term dermal tolerability and laboratory repellency of MosShield, a ready-to-use topical spray formulated with 6% w/w Litsea cubeba essential oil. Non-confidential formulation quality-control parameters, including appearance, homogeneity, pH, density, and apparent viscosity, were recorded to support formulation consistency before testing. Short-term dermal tolerability was assessed using a human skin-patch test in 48 healthy volunteers, with no visible skin reactions observed up to 96 h after application. Repellency assays were conducted following the World Health Organization arm-in-cage protocol against three medically important mosquito species: Aedes aegypti, Anopheles dirus, and Culex quinquefasciatus. The formulation showed species-dependent repellency, achieving median complete protection times of 225 min against Cx. quinquefasciatus and 120 min against both Ae. aegypti and An. dirus. These findings provide product-relevant laboratory evidence supporting further evaluation of the 6% L. cubeba essential oil spray as a plant-based topical mosquito repellent under semi-field and field conditions.

PMID:
42449186
Bibliographic data and abstract were imported from PubMed on 15 Jul 2026.

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