Authors
Meng Zhang, Yong-Li Li, Guang-Wei Zhang, Yi Song, Ying Cui, Yan-Fen Li, Hao-Yu Qi
Published in
International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases. Pages 108976. Jul 14, 2026. Epub Jul 14, 2026.
Abstract
To complete the traceability from patient to food using SNP-based clustering analysis technology after whole genome sequencing in botulinum toxin poisoning incidents.
Botulinum toxin in patient's blood and urine were detected, real-time PCR was used for preliminary screening and typing of Clostridium botulinum toxin genes in patient's feces and all suspicious samples after epidemiological investigation, then antitoxin serum was treated to patient within 2 h Clinically. Finally, SNP-based clustering analysis achieved traceability from patient to food at the genetic level.
Botulinum toxins in patient's blood and urine were positive, confirming food borne botulinum toxin poisoning. Virulence genes of type B were detected in patient's feces and homemade sufu sample. Mouse intraperitoneal injection test confirmed homemade sufu contained botulinum toxin. The isolated Clostridium botulinum showed typical tennis racket shape under microscope, MLST type was ST115, and neurotoxin (BoNT) was type B1. After whole genome sequencing, SNP-based clustering analysis found that the difference number of SNP site between strains of Clostridium botulinum from feces and sufu was 0.
This technology traced the etiological food and excluded other factors unrelated to the poisoning incident. Prevent the recurrence of similar poisoning incidents caused by the same batch of food.
PMID:
42448164
Bibliographic data and abstract were imported from PubMed on 15 Jul 2026.
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