Authors
Qiwen Shi, Haiping Xiang, Ying Fan, Chunyan Wu, Shengqiang Tong
Published in
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences. Volume 1281. Pages 125210. Jul 11, 2026. Epub Jul 11, 2026.
Abstract
This study aimed to isolate and characterize α-glucosidase inhibitory and antioxidant compounds from the roots of Lygodium japonicum and to investigate their interactions with α-glucosidase. Dual high-resolution α-glucosidase inhibition and 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radical scavenging profiling was employed, which led to the identification of 12 constituents associated with α-glucosidase inhibitory and/or antioxidant activities from L. japonicum roots, including two new compounds, 6-hydroxy-7-(hydroxymethyl)-2-isopropyl-1,4-naphthoquinone (7) and 1,1'-dimer of 2,3-dihydroxy-4-methoxydibenzofuran (10). Several isolated compounds showed notable bioactivities, with compounds 7, 8, and 10 exhibiting measurable α-glucosidase inhibitory activity (IC50 < 11 μM) and compounds 1, 2, 4, 5/6, and 10 displaying robust DPPH radical scavenging activity (IC50 < 20 μM). Enzyme inhibition kinetics, fluorescence spectroscopy, circular dichroism spectroscopy, and molecular docking were employed to characterize three of the most potent α-glucosidase inhibitors and to reveal their potential binding interactions with the enzyme. Overall, this study highlights the utility of dual high-resolution bioactivity profiling for identifying chemically defined constituents that contribute to the in vitro α-glucosidase inhibitory and antioxidant activities of L. japonicum roots.
PMID:
42456228
Bibliographic data and abstract were imported from PubMed on 16 Jul 2026.
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