A novel dual-mode Fluorescence/Colorimetric Aptasensor Based on NH₂-MIL-88B(Fe)/CDs-Apt for Kanamycin Detection.

Authors

Huijing Hou, Mingming Luo, Fang Li, Yijun Tan, Ruowei Liu, Longping Xu, Yanwen Feng, Xiangli Guo, Zijian Wu

Published in

Journal of fluorescence. Jul 17, 2026. Epub Jul 17, 2026.

Abstract

Kanamycin (KANA) is a veterinary drug residue that is highly scrutinized in food safety testing. In this study, a metal-organic framework-based aptamer-nanoenzyme (NH₂-MIL-88B(Fe)/CDs-Apt) was introduced for the dual-mode detection of KANA. NH₂-MIL-88B(Fe) catalyzes the oxidation of 3,3',5,5'-tetramethylbenzidine (TMB) by H₂O₂ to form blue ox-TMB, thereby enabling colorimetric detection. As a fluorescence quencher, NH₂-MIL-88B(Fe) quenches the fluorescence of CDs via photogenerated electron transfer (PET) or fluorescence resonance energy transfer (FRET) mechanisms. The detection limits were 0.716 fM (fluorescent) and 0.709 fM (colorimetric), respectively. The proposed aptasensor was successfully applied to the detection of KANA in eggs, honey, and lake water. This aptasensor demonstrates high sensitivity and accuracy, as well as broad application potential.

PMID:
42467307
Bibliographic data and abstract were imported from PubMed on 17 Jul 2026.

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